The pectic enzymes of Aspergillus niger. 2. Endopolygalacturonase
نویسندگان
چکیده
منابع مشابه
The active site topology of Aspergillus niger endopolygalacturonase II as studied by site-directed mutagenesis.
Strictly conserved charged residues among polygalacturonases (Asp-180, Asp-201, Asp-202, His-223, Arg-256, and Lys-258) were subjected to site-directed mutagenesis in Aspergillus niger endopolygalacturonase II. Specific activity, product progression, and kinetic parameters (K(m) and V(max)) were determined on polygalacturonic acid for the purified mutated enzymes, and bond cleavage frequencies ...
متن کاملSubsite mapping of Aspergillus niger endopolygalacturonase II by site-directed mutagenesis.
To assess the subsites involved in substrate binding in Aspergillus niger endopolygalacturonase II, residues located in the potential substrate binding cleft stretching along the enzyme from the N to the C terminus were subjected to site-directed mutagenesis. Mutant enzymes were characterized with respect to their kinetic parameters using polygalacturonate as a substrate and with respect to the...
متن کاملPectic Enzymes
Indoleacetic acid (IAA) and/or inhibitors of DNA, RNA or protein synthesis were added to the apex of decapitated seedlings of Pisum sativum L. var. Alaska. At various times up to 4 days, enzymic protein was extraoted from a segment of epicotyl immediately below the apex and assayed for its ability to hydrolyse polysaccharides or their derivatives. With the exception of am;ylase, the total amoun...
متن کاملProduction of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger
A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h. The...
متن کاملDegradation of pectic polysaccharides in various fruits by pectinase derived from Aspergillus niger
Aspergillus spp. are widely spread plant pathogens. In several kinds of fruit, especially in apple and peach, aspergillus rot is one of the postharvest desease in fruit, but the infection mechanism is not known. The degradation of cell walls by pathogens is significant in primary infection and afterinfection, and plays an important role in the advancement of mycelium into the host plant and the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1961
ISSN: 0306-3283
DOI: 10.1042/bj0790057